Organogenesis from cotyledon and hypocotyl-derived explants of japhara (Bixa orellana L.)

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been developed with significant organogenic response from rooted hypocotyls, hypocotyl segments, and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg L thidiazuron and 0.25% coconut water or 7.0 mg L N-Benzyladenine and 0.1 mg L a-naphthalene acetic acid. Thidiazuron in combination with coconut water promoted higher organogenic response in rooted hypocotyls. Similarly direct organogenesis was noticed from hypocotyls, cotyledonary leaf explants and shoot tip explants on half MS medium with 0.25 mg L N-Benzyladenine (BA) and 0.5 mg L indole-3-acetic acid (IAA). Best shoot elongation of shoot buds was achieved in the presence of 1.5 mg L (BA) + 1.0 mg Lindole-3-butyric acid (IBA). The in vitro rooting of microshoots was good in the presence of 3.0 mg L (IBA). Seventy percent of rooted plants survived after acclimatization.